FastQCFastQC Report
水 28 3 2018
TL10_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameTL10_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences187577883
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCT7101710.37860060506173854No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGTAC418750.010.0581819
GCCATAC473050.08.9081063
GGCCATA493000.08.9017162
AGCACAC1307700.08.7200459
ATCGGAA1415350.08.209221
AGAGCAC1564150.07.38329277
ACCCTAA15196550.07.33802564
AACCCTA15345250.07.29539733
CCTAACC15504900.07.23419486
CCCTAAC15581550.07.19952445
GAGCACA1634150.07.1688188
TAACCCT16164450.06.96280342
CTAACCC16766400.06.73591661
CATACTA707000.06.67534165
TCGGAAG1792650.06.6563432
GGGATCC754750.06.21752363
CGGAAGA1983400.06.0155023
GGGCCAT748050.05.96198271
ACTAGTA841200.05.54127658
TACTAGT865350.05.47896677
Produced by FastQC (version 0.11.5)