FastQCFastQC Report
水 28 3 2018
TL10_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameTL10_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences187577883
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCT6568090.35015268831027374No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGTAC436800.09.0449359
GGCCATA491850.08.027132
GCCATAC487800.07.9557983
ACCCTAA14523850.07.5904934
AACCCTA14673550.07.5255263
CCTAACC14861150.07.4501776
CCCTAAC14974350.07.40829325
TAACCCT15312100.07.2628498
CTAACCC16145650.06.97325231
ATCGGAA1738700.06.73911761
GGGATCC761050.06.1191863
CATACTA713700.05.941965
TCGGAAG2105400.05.6705522
GGGCCAT742850.05.538541
TACTAGT835850.05.25580177
CCATACT866500.05.24885134
ACTAGTA839250.05.1653288
CGGAAGA2368850.05.08632233