FastQCFastQC Report
火 27 3 2018
TL9_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameTL9_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences180128577
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCT6062490.3365645863065914No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC1285750.013.1032999
ATCGGAA1392000.012.3390331
AGAGCAC1528350.011.1660217
GAGCACA1616400.010.67853458
TCGGAAG1715700.010.1848662
CTAGTAC403900.09.4229429
CGGAAGA1950100.08.9113093
GCCATAC449950.08.5754913
GGCCATA461550.08.5180542
ACCCTAA12694600.07.50039534
AACCCTA12838900.07.43598753
CCTAACC12997450.07.3709566
CCCTAAC13089450.07.3036315
TAACCCT13580100.07.0827262
CTAACCC14225750.06.82138351
GTATGCC562800.06.64958445-49
AAGAGCA2820650.06.50515656
CATACTA674150.06.1618675
GGGATCC728450.06.0237543
CGTATGC646800.05.866705445-49
Produced by FastQC (version 0.11.5)