FastQCFastQC Report
火 27 3 2018
TL9_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameTL9_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences180128577
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCT5546380.307912275352067No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1740450.010.1514231
TCGGAAG2092750.08.5413092
CTAGTAC415900.08.2620019
ACCCTAA12020800.07.67787274
GGCCATA471250.07.66929242
AACCCTA12176700.07.6150149
CGGAAGA2352600.07.6033253
CCTAACC12336800.07.52621846
CCCTAAC12439000.07.4689515
TAACCCT12775100.07.3237482
GCCATAC474750.07.31861973
AGAGCGT2523600.07.1815387
CTAACCC13579150.06.9624051
GAGCGTC2727100.06.64021838
AGCGTCG3138350.05.9249119
GGGATCC717400.05.6622013
CATACTA680650.05.59149655
AAGAGCG3602300.05.27253446
GGGCCAT712950.05.26389741
TACTAGT797000.05.0572517
Produced by FastQC (version 0.11.5)